J. Anim Sci.
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J. Anim Sci. 1968. 27:1049-1053.
© 1968 American Society of Animal Science
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Comparison of Metabolites of Progesterone-4-14C in Urine from a Nonpregnant Domestic Sow1

Patricia H. Jones2 and R. E. Erb

Purdue University, Lafayette, Indiana

Abstract

Urine excreted following intravenous administration of 9 µc. representing 166 mcg. of progesterone-4-14C to one sow on day 13 and day 19 of the estrous cycle was used to evaluate extraction procedures and to characterize the steroid compounds containing 14C. Approximately 80% of the radioactivity in urine was rendered extractable by sequential treatment with ß-glucuronidase enzyme and solvolysis. An additional 17% was extracted after treatment of the latter aqueous residue with acid which indicates the presence of conjugates other than simple glucuronides or sulfates.

A major portion of the extracted radioactivity was in the neutral fraction (89%) as compared with 6.6% in the acid+phenolic and 4.3% in the petroleum ether-benzene phenolic fractions. Four major metabolites of progesterone-4-14C, previously identified, account for 80 to 90% of the radioactivity in the neutral fraction. Four other unidentified 14C metabolites were isolated but these represent only a small portion of the metabolites of progesterone in sow urine.

Footnotes

1 Journal Paper No. 3241, Purdue University Agricultural Experiment Station. A contribution from the Department of Animal Sciences, financed in part by the Purdue Research Foundation Grant No. 4291.

2 A Fellow, Purdue Research Foundation. Present address: Department of Zoology, Ohio University, Athens.

4 Endo Laboratories, Garden City, New York.

5 Warner Chilcott, Morris Plains, New Jersey.






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Copyright © 1968 by the American Society of Animal Science.