J. Anim Sci.
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J. Anim Sci. 1968. 27:497-504.
© 1968 American Society of Animal Science
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Metabolism of 75Se-Selenite, 75Se-Selenate,75Se-Selenomethionine and 35S-Sulfate by Rumen Microorganisms in vitro1

G. D. Paulson2, C. A. Baumann and A. L. Pope

University of Wisconsin3, >Madison

Abstract

Studies were conducted to compare the metabolism of 75Se-selenomethionine, 75Se-selenite,75Se-selenate and 35S-sulfate by rumen microorganisms in vitro. There was a gradual incorporation of 35sulfur activity into the TCA-insoluble fraction of the rumen fluid which could be inhibited by excess sulfate. The incorporation of 35sulfur into methionine was confirmed by paper and ion exchange chromatography. There was a gradual incorporation of 75Se-selenomethionine into the TCA-insoluble fraction of the rumen fluid which could be inhibited by excess methionine;Pronase released most of the activity from the TCA-insoluble fraction, approximately 60% of which was identified as 75Se-selenomethionine by paper and ion exchange chromatography. Radioactivity added to the rumen fluid as 75Se-selenite and 75Se-selenate was rapidly incorporated into the TCA-insoluble fraction. However, most of the activity added as inorganic selenium was apparently loosely bound and could be removed by dialysis when reduced glutathione was in the dialysis bath; the binding of selenite was not enzyme dependent. The incorporation of inorganic selenium into the TCA-insoluble fraction was not inhibited by excess sulfate, and very little or none of the activity added to the rumen fluid as 75Se-selenite or 75Se-selenate was incorporated into 75Se-selenomethionine.

Footnotes

1 Published with the approval of the Director of the Wisconsin Agricultural Experiment Station. Supported in part by funds from the Se-Te Development Committee.

2 Present address: U.S.D.A., A.R.S., Metabolism and Radiation Research Laboratory, State University Station, Fargo, North Dakota 58102.

3 Biochemistry and Animal Science Departments.






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Copyright © 1968 by the American Society of Animal Science.