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Diet and Supplemental Enzyme Effects on Volatile Fatty Acids of Bovine Rumen Fluid¹

Washington State University, Pullman

Abstract

Four rumen fistulated cattle were used in a series of experiments to determine the effect of enzyme supplementation upon their rumen fluid's volatile fatty acid composition. The effects of diet, sampling time, and individual animal upon the volatile fatty acid composition also were determined.

The effect of the enzymes was detected only when the animals were fed diets containing either 50% or 80% of their diet as a concentrate mixture (66.7% steam rolled barley); no effect was found with a 100% alfalfa hay diet. The diet, sampling time, and individual animals each had significant effects upon the volatile fatty acid concentration of the rumen fluid.

The molar proportions of acetic acid in the rumen fluid was reduced and the percentage of propionic or butyric acid increased when supplementary enzymes were fed with high grain rations.

¹ Scientific Paper No.2787, College of Agriculture, Washington State University, Pullman, Washington. Project 1217.

² Appreciation is expressed to Rohm and Haas Co., Washington Square, Philadelphia 5, Pennsylvania, for financial support of this work and the Rhozyme F 3C; and to Dr.T. S. RusSell for assistance with the statistical analvses.

³ These data are a portion of a Ph.D. thesis by the senior author, Washington State University.