HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Plotka, E. D. Articles by Erb, R. E. Search for Related Content PubMed PubMed Citation Articles by Plotka, E. D. Articles by Erb, R. E.

Levels of Progesterone in Peripheral Blood Plasma during the Estrous Cycle of the Ewe^{1, 2,}

Purdue University, Lafayette, Indiana

Abstract

Fifty peripheral venous blood samples were collected from 37 ewes in different stages of the estrous cycle to estimate changes in progesterone concentration. After separation of the plasma, progesterone levels were estimated by a double-isotope derivative method involving conversion of progesterone to 20 ß-hydroxy-pregnene-3-one with sodium boro-hydride-³H. Progesterone levels increased from 22.2°2A ng./ml. (1O^–9 gm./ml.) around estrus to 37.4°4.6 ng./ml. by 9 to 11 days (P<.01), and then declined to 21.8°3.0 ng./ml. 13 to IS days after estrus (P<.01).The average for the experiment was 27.7°5.2 ng./ml. with an average coefficient of variability of 41.7 %.

¹ Journal Paper No.3008. Purdue University Agricultural Experiment Station, 47907.

² Department of Animal Sciences.

³ Acknowledgment is due Doris Blair and Janice Lycette for their laboratory assistance.