J. Anim Sci.
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J. Anim Sci. 1967. 26:1104-1107.
© 1967 American Society of Animal Science
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Sodium and Potassium Content of Bull Spermatozoa during Processing Prior to Freezing1

A. M. Yassen2, 3, and R. H. Foote4

Cornell University, Ithaca, New York

Abstract

The average values in mcg./ml. for the whole bull semen, spermatozoa and seminal plasma for Na were 1,678, 1,027 and 1,768; corresponding values for K were 1,187, 1,887 and 1,095. The Na and K concentration in semen varied greatly between bulls. Extending the semen with Tris-buffered egg yolk at room temperature caused the cells to outflux 85% of their Na and 75% of their K within 15 min. after extension. This resulted in an equilibrium between intra- and extracellular Na, but intracellular K remained three times as high as K in the extracellular fluid.

When spermatozoa were extended and stored at room temperature or cooled to 5° C, only moderate changes took place following the decrease of intracellular Na and K at the time of extension. Na and K levels tended to rise (P<.05) during cooling and storage except when glycerol was added. Intracellular K concentration was maintained at a higher level than intracellular Na during a 4-hr, period, but neither ion concentration was as high as intracellular levels in fresh bull semen.

Footnotes

1 The Authors are Grateful for the Support Given by the Eastern Artificial Insemination Cooperative, Inc.

2 Supported by a Government Fellowship From the U.A.R.

3 Present Address: Animal Production Department, College of Agriculture, Alexandria University, Alexandria, U.A.R.

4 Department of Animal Science.






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Copyright © 1967 by the American Society of Animal Science.