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Free Amino Acids in Porcine Spermatozoa¹

Purdue University, Lafayette, Indiana

Abstract

The free amino acids in spermatozoa from two ejaculates from each of six mature Yorkshire boars were determined by ion exchange chromatography using a Dowex 50-x 12 resin. Twenty amino acids were identified by comparison with standards and by peak enhancement, and were quantified as the percentage each contributed to the total amino acids in a semen sample. Glutamic acid was the major amino acid in each sample, averaging 23.8% compared to 7.3% for serine, the second most prevalent amino acid. Aspartic acid, taurine, lysine and threonine averaged from 6.5 to 5.9% in descending order, respectively. Similarly arginine, leucine, proline, alanine and glycine averaged from 5.5 to 4.6%. Ornithine, valine and isoleucine averaged from 3.6 to 3.0%. Cysteic acid, phenylalanine, tyrosine, histidine, cysteine and methionine averaged from 2.3 to 0.5%. In addition, an unidentified peak of variable shape and larger than the glutamic acid peak was observed. Differences among boars or ejaculates within boars were not significant for any of the amino acids.

¹ Journal Paper No. 3,002, Purdue University Agricultural Experiment Station, 47,907. A Contribution from the Department of Animal Sciences.

² Acknowledgment is due W. R. Featherston for his technical assistance and advice.