J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J. Anim Sci. 1967. 26:1072-1077.
© 1967 American Society of Animal Science
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Foley, C. W.
Right arrow Articles by Erb, R. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Foley, C. W.
Right arrow Articles by Erb, R. E.

Effects of Zero and Three-Day Storage of Washed Boar Spermatozoa on Subsequent Incubation Characteristics

C. W. Foley, H. M. Marsh, C. J. Heidenreich1, V. A. Garwood and R. E. Erb

Purdue University, Lafayette, Indiana2

Abstract

Two experiments were conducted with washed resuspended boar spermatozoa. In Experiment I, one part spermatozoa was resuspended in five parts of glucose extender and anaerobically incubated for 12 hr. at 37° C, during which time lactic acid, glucose, pH and motility change were measured. Lactic acid accumulation continued throughout incubation. Highly significant differences between pooled ejaculates were observed for lactate accumulation. Glucose content and motility declined steadily through incubation time. In Experiment II, one part spermatozoa was resuspended in six parts extender (either a glucose or fructose extender) and incubated aerobically or anaerobically. Fresh and spermatozoa aged for 72 hr. were studied. Lactic acid, pH and motility changes and oxygen consumption were measured.

When analyzed on a time-interval basis (actual accumulation per hr.), the amount of lactic acid produced through incubation by fresh spermatozoa was not significantly different from the amount produced by spermatozoa after 72-hr. aging. The lactic acid accumulation (fresh spermatozoa) was greater (P<.01) in the glucose than in the fructose extender. The pH was higher (P<.01) in the glucose extender than in the fructose extender. Highly significant differences were observed for pH between aerobic and anaerobic incubation conditions. Motility was maintained at a higher (P<.01) level in the glucose extender than in the fructose extender. Extenders did not significantly influence Oa consumption of the resuspended spermatozoa. Fresh spermatozoa consumed more (P<.01) than aged spermatozoa.

Footnotes

1 Present Address: Wisconsin State University, Platteville.

2 Contribution from the Department of Animal Science, Purdue University Agricultural Experiment Station, Journal Paper No. 2868.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1967 by the American Society of Animal Science.