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Rates of In Vitro Cellulose and Dry Matter Digestion at 5, 8 and 11 Hours as Predictors of Forage Nutritive Value^{1, 2,}

Ohio Agricultural Research and Development Center, Wooster³

Abstract

Preliminary experiments were conducted to determine the in vitro fermentation time periods when measurements should be made to estimate maximum digestion rate. Using cellulose digestion and dry matter disappearance as criteria, it was established that with the system employed, this period occurred between 5 and 11 hours. These two measurements were then made on a series of 65 forage samples at 5, 8 and 11 hours. Digestibility values at each time interval and the rate of digestion values, calculated for the intervals 5 and 8, 8 and 11 and S and 11 hr. were correlated with in vivo digestibility data. The correlation coefficients obtained with the rate data were of the same magnitude as those obtained with in vitro data taken at a specific time.

¹ Approved for publication as Journal Article No. 99-65 by the Associate Director of the Ohio Agricultural Research and Development Center, Wooster, Ohio.

² This work was supported in part by funds allocated to the Center on the recommendation of the North Central Technical Committee on The Development and Application of Laboratory Methods for Determining Forage Quality (NC-64).

³ Department of Animal Science.