J. Anim Sci.
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J. Anim Sci. 1967. 26:302-306.
© 1967 American Society of Animal Science

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Some Sources of Variation Associated with Ether Extraction1

R. W. Mandigo2, A. W. Munson3, R. L. Henrickson4 and J. V. Whiteman4

Oklahoma Agricultural Experiment Station, Stillwater

Abstract

Four hundred and thirty-two determinations of ether extractable fat content of beef samples were made. Duplicate determinations at three extraction periods (12, 18 and 24 hr.) on two apparatus repeated six times for each of six levels of fat content were obtained. Each of the six levels was, therefore, determined using 72 aliquots from a ground, homogenized and thoroughly mixed sample. The lean and fat from beef flanks were blended to produce fat contents of 3, 18, 30, 43, 57 and 76%. Extraction time was found to be the most important source of variation associated with the determination. The effect of extraction time on low levels of fat content (3, 18, 30 and 42%) appears to have little influence on the magnitude of the error. At higher levels of fat (57 and 76%) highly significant (P<.01) differences in the errors were found for extraction periods of less than 24 hours. Variances were found to increase greatly with the increasing fat content, and longer extraction only partially reduces the magnitude. The basic technique discussed can be applied to many procedures and apparatus to provide greater knowledge in reference to the precision of the results obtained, and thus the confidence that can be placed in the data.


Footnotes

1 Approved as Journal Manuscript No. 1321 of the Oklahoma Agricultural Experiment Station.

2 Present address: Animal Science Department, University of Nebraska, Lincoln.

3 Present address: Biometrical Service, U.S.D.A., Beltsville, Maryland.

4 Institute of Animal Sciences and Industry, Stillwater, Oklahoma.







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Copyright © 1967 by the American Society of Animal Science.