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Effect of Aging of Boar Spermatozoa on Cellular Loss of DNA^{1, 2,}

University of Wisconsin, Madison³

Abstract

The DNA content of spermatozoa from pooled spermatozoa-rich fractions of two boars was studied in eight trials using chemical analysis with the diphenylamine reaction and purified Salmon sperm cell DNA as a standard. The DNA content was quantitated at zero hour (fresh spermatozoa) and after 24 hr. of aerobic storage at 37° C. in a phosphate buffer containing glucose (aged spermatozoa). The DNA content of boar spermatozoa decreased significantly during 24 hr. of storage. The average decrease was 15.4%. A successive decrease in the DNA content of the sperm cells was observed between trials as the trials progressed, but the biological significance of this observation is unknown. The decrease in DNA upon ^{in vitro} aging of the cells may be related to the known decrease in fertility of aged spermatozoa, as well as the apparent increase in embryonic mortality resulting from the use of aged spermatozoa in artificial insemination.

The average DNA content of a fresh boar spermatozoon was 3.6X10^–9 mg.

¹ Published with the approval of the Director of the Wisconsin Agricultural Experiment Station, Madison.

² This study was supported in part by grants from the American Meat Institute, Tri-State Breeders Cooperative, Badger Breeders Cooperative and the Murphy Products Company.

³ Department of Meat and Animal Science Paper No. 452 and Department of Biochemistry, cooperating.