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University of Florida, Gainesville3
Abstract
The effects of varying dietary levels of P, S and Mo upon the levels of P, S and Cu in the rumen fluid during 32 in vivotrials were determined. Two experiments were carried out with four steers during eight consecutive 25-day periods. The first trial considered steer uniformity, and the second trial involved mineral interrelationships using a factorial design. Twenty-five-day pretrial P levels of 0.04, 0.16 and 0.54% did not exert carryover effects during the following 25-day trial periods with 0.04, 0.16 and 0.54% dietary P. These dietary P levels resulted in 0.87, 2.30 and 2.87% P in the rumen fluid dry matter or 198, 417 and 543 mcg. per ml. of rumen fluid (r=0.77, P<.01). Rumen fluid P tended to plateau just above 0.16% dietary P or at 31 mg. per kg. of body weight. Below 0.16% of dietary P both the animal and the rumen microorganisms reflected a P deficiency. With dietary P, S and Mo levels of 0.16, 0.15 and 0.00015%, respectively, rumen fluid levels of S and Cu were 0.26 and 0.0004% in the dry matter and 49 and 0.07 mcg. per ml. in the rumen fluid, respectively. By increasing dietary S as Na2SO4 to 0.55%, S increased to 0.41% and 91 mcg. per ml. in the dry matter and liquid of the rumen fluid, respectively. P was concentrated in the rumen fluid to a greater degree than S.
1 Florida Agricultural Experiment Stations Journal Series No. 2156
2 Present address: Department of Animal Sciences, Rutgers University, New Brunswick, New Jersey.
3 Nutrition Laboratory, Department of Animal Science.
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