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Iowa State University, Ames
Abstract
The addition of washed rumen protozoa to a washed suspension of rumen bacteria substantially increased cellulose digestion and acid production. The molar proportion of acetic acid was decreased, and butyric acid increased when both types of microorganisms were present. About 70% of the stimulatory activity of protozoa upon cellulose digestion by rumen bacteria was retained following autoclaving of the protozoa. Additions of protozoa increased cellulose digestion by rumen bacteria cultured in vitro in the presence of added volatile acids, a combination of vitamins B12 and biotin or acid hydrolyzed casein.
1 Journal Paper No. J-5101 of the Iowa Agricultural and Home Economics Experiment Station, Ames, Iowa. Project No. 1526, contributing project to Regional Research Project NC 63.
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