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Progesterone Levels in Ovarian Venous Effluent of the Nonpregnant Sow^{1, 2, 3,}

Purdue University, Lafayette, Indiana

Abstract

Seventy-four ovarian venous blood samples were obtained from 18 sows by cannulation of an ovarian vein and collection of blood.

Progesterone levels in blood plasma samples were measured by a double-isotope derivative method involving conversion of progesterone to 20ß-hydroxy-⁴-pregnene-3-one (20ß-ol) with sodium borohydride-³H.

Progesterone levels increased from 0.03± 0.01 meg./ml. of plasma on the first day of estrus to 1.07±0.13 meg./ml. on days 10 to 12 of the estrous cycle. Thereafter, the level decreased to 0.04±0.01 meg./ml. on days 19 to 21 of the cycle.

Two ultraviolet absorbing compounds other than progesterone were found in the plasma samples. These probably were not 20ß-ol or its 20_a-epimer, 17_a-hydroxyprogesterone, androstenedione or pregnenolone.

¹ Journal Paper No. 2517, Purdue University Agricultural Experiment Station.

² Contribution from Departments of Animal Sciences and Veterinary Clinics and Medicine.

³ Data taken from a thesis submitted by the senior author to the Graduate School, Purdue University, in partial fulfillment of the requirements for the Ph.D. degree.

⁴ Present address: Department of Dairy Science, Ohio State University, Columbus, Ohio.

⁵ Present address: Methodist Hospital, Indianapolis, Indiana.