J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J. Anim Sci. 1965. 24:347-354.
© 1965 American Society of Animal Science
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Desjardins, C.
Right arrow Articles by Hafs, H. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Desjardins, C.
Right arrow Articles by Hafs, H. D.

Immunochemical and Electrophoretic Properties of Equine Luteinizing Hormone1

Claude Desjardins and Harold D. Hafs

Michigan State University, East Lansing2

Abstract

Antiserum to equine LH was produced in 10 rabbits to determine the extent to which it would cross-react with LH from other species and with other pituitary hormones.

Antiserum, after absorption with equine blood serum, resulted in a single precipitation when diffused against as little as 0.8 µ. equine LH in agar gel. This precipitation line for equine LH was confluent with a similar line for PMS and with similar lines for saline extracts of bovine, ovine, and rat pituitaries, respectively. The antiserum failed to react with other hormones purified from the anterior pituitary, with HCG or with saline extract of chicken pituitaries. Immunoelectrophoresis and complement fixation tests generally substantiated the conclusion from agar gel double diffusion studies that antiserum to equine LH reacted with each substance that was expected to contain appreciable quantities of LH.

Ovarian ascorbic acid depletion asays of purified equine, bovine and ovine LH as well as saline extracts of bovine, ovine, and rat pituitaries and mixtures of each of these sources of LH with antiserum to equine LH revealed that the antiserum neutralized the physiological activity of each of these sources of LH.

Footnotes

1 Journal Article No. 3460 from the Michigan Agricultural Experiment Station. The authors acknowledge generous supply of hormones from the Endocrinology Study Section, National Institutes of Health.

2 Animal Reproduction Laboratory, Dairy Department.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1965 by the American Society of Animal Science.