J. Anim Sci.
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J. Anim Sci. 1965. 24:156-160.
© 1965 American Society of Animal Science
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Supplementation of Dairy Calf Diets with Enzymes. II. Effect on Nutrient Utilization and on Composition of Rumen Fluid1,2,

J. W. Rust3, N. L. Jacobson, A. D. McGilliard and D. K. Hotchkiss4

Iowa State University, Ames

Abstract

The apparent nitrogen and energy digestibilities and nitrogen retention were determined for a hay and concentrate ration with and without bacterial protease concentrate supplementation, using 6- to 7-month-old steers. Samples of rumen fluid were analyzed for VFA, total nitrogen, soluble nitrogen, NPN and ammonia nitrogen at 0, 1, 2, 4 and 6 hr. after feeding each ration.

Enzyme supplementation significantly increased apparent nitrogen and energy digestibility. There was no apparent effect on total concentration or on the ratios of VFA in rumen fluid. Protein nitrogen concentration was significantly higher for the supplemented ration at 0, 2, 4 and 6 hr. after feeding, but lower at 1 hr. after feeding. Non-ammonia NPN concentration was significantly higher at 1 hr. after feeding for the supplemented ration than for the control. There were no significant differences between rations in ruminal ammonia concentration.

Footnotes

1 Journal Paper No. J-4691 of the Iowa Agricultural and Home Economics Experiment Station, Ames, Iowa. Project No. 1324.

2 Supported in part by funds provided by Miles Chemical Company, Elkhart, Indiana, which also supplied the enzyme supplement.

3 Present address: Department of Dairy Husbandry, University of Minnesota, St. Paul.

4 The authors wish to acknowledge the technical assistance of Mrs. Marlene Richard, Mrs. Alice Schmidt, John M. Bryant and Mrs. Lillian Kraft.






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Copyright © 1965 by the American Society of Animal Science.