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Absorption of Vitamin B₁₂ from the Colon of the Pig^{1, 2,}

Ohio Agricultural Experiment Station, Wooster

Abstract

Six pigs were provided with colon cannulas introduced into the first portion of the colon via the cecum. Vitamin B₁₂ labeled with Co⁵⁷ was introduced into the colon. By the collection of feces and urine during a 7-day period, together with analysis of organ tissue, it was demonstrated that 41.6 to 58.0% of the radioactive cobalt was absorbed. Based upon the charcoal adsorption of radioactivity present in the feces, it appeared that some vitamin B₁₂ was metabolized during its passage through the lower digestive tract.

¹ Approved for publication as Journal Article No. 8–64 by the Associate Director of the Ohio Agricultural Experiment Station, Wooster.

² This study was supported in part by a grant from American Cyanamid Company, Princeton, New Jersey. The labeled vitamin B₁₂ was supplied by Merck and Co., Inc., Rahway, New Jersey.

³ Postdoctoral fellow. Present address: Department of Nutrition, State Agricultural College, Ghent, Belgium.