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Mucopolysaccharide Excretion in Dwarf and Normal Cattle¹

Michigan State University, East Lansing, and University of California, Davis

Abstract

Excretion of mucopolysaccharides has been measured in dwarf and normal cattle and in patients with Hurler's disease. Total mucopolysaccharides were determined by the carbazole uronic acid test. Chondroitin sulfates were determined by a chondroitinase assay. Chondroitin sulfate B was estimated by the carbazole and naphthoresorcinol reactions.

When mucopolysaccharides in the urine of cattle are expressed relative to creatinine output, there is a well denned pattern characterized by a decrease in mucopolysaccharide excretion with advancing age of the animal. After making appropriate allowances for age and creatinine output, mucopolysaccharide excretion was found to be similar for dwarf and normal cattle by all qualitative and quantitative tests used. Gross and microscopic examinations at autopsy of dwarf cattle revealed differences in tissues from those reported for patients with Hurler's disease.

¹ Journal Article No. 3171 of the Michigan Agricultural Experiment Station. Supported by research grants (AM-05321 and GM-09266) from the USPHS.

² Department of Biochemistry, Michigan State University, East Lansing. Predoctoral fellow of the USPHS (GM-18,387).

³ Department of Biochemistry, Michigan State University, East Lansing.

⁴ Department of Animal Husbandry, University of California, Davis.

⁵ Department of Anatomy, School of Veterinary Medicine, University of California, Davis.

⁶ The authors acknowledge with thanks Mrs. Susan Kent for technical assistance, S. J. Sanfilippo, University of Minnesota, for supplying the urine from Hurler's patients and E. J. Turman, Oklahoma State University, for supplying the tissue samples from a snorter dwarf calf for the preliminary experiments.

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