J. Anim Sci.
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J. Anim Sci. 1964. 23:67-70.
© 1964 American Society of Animal Science
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Effect of Osmotic Pressure on Boar Spermatozoa1,2,

E. J. Stevermer3, N. L. First and W. G. Hoekstra

University of Wisconsin, Madison4

Abstract

Two experiments were conducted to study the influence of osmotic pressure on boar spermatozoa motility preservation in skimmilk diluents. A third experiment was conducted to determine its influence on fertility.

When meso-inositol was used to adjust osmotic pressure, highest motility ratings were obtained in diluents with freezing points between –.400° and –.500° C. Higher and lower levels reduced motility. When D-fructose was used, no differences in motility were observed in diluents with freezing points ranging from –.400° to –.550° C; however, lower levels were deleterious.

Within any one osmotic pressure group, motility preservation was significantly (P<.05) better when the amount of skimmilk in the diluent was between 5% and 8%.

On the basis of 48-hour storage, boar spermatozoa fertility was preserved better in diluents with freezing points of –.535° C. compared with –.410° or –.475° C. These data indicate that motility of stored spermatozoa is not a reliable indicator of spermatozoa fertility.

Footnotes

1 Published with the approval of the Director of the Wisconsin Agricultural Experiment Station, Madison.

2 This study was supported in part by grants from Oscar Mayer and Company, Tones Dairy Farm and The Wisconsin-Minnesota Cooperative Bull Studs.

3 Present address: Animal Science Department, Iowa State University, Ames, Iowa.

4 Department of Meat and Animal Science (Paper No. 371) and Department of Biochemistry cooperating.






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Copyright © 1964 by the American Society of Animal Science.