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Properties of a Slime Isolated from the Rumen Fluid of Cattle Bloating on Clover Pasture¹

United States Department of Agriculture^,3 and State University of Mississippi^,4

Abstract

Ethanol precipitated slime fractions have been isolated from centrifuged rumen fluid of steers bloating on clover pastures. The amount of slime increased during grazing when the animals bloated. Analysis showed the fractions contained 61–64% protein, 8–14% carbohydrate and 7–10% ribonucleic acid. The slime fraction contained 17 amino acids, the sugars d-ribose, arabinose, and glucose and the purines and pyrimidines adenine, guanine, cytosine and uracil. The possible relationship of the slime fractions to bloat is discussed.

¹ This manuscript is published with the approval of the Director of the Mississippi Agricultural Experiment Station, State College, as Journal paper number 1048.

² The authors wish to express their appreciation to C. B. Shawyer, L. W. Williams and L. Waymack for assistance during the course of the experiments.

³ Animal Husbandry Research Division, Agricultural Research Service, Beltsville, Maryland.

⁴ Animal Husbandry Department.

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