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Reciprocal Transfer of Cattle and Rabbit Embryos^1,2,3,

Washington State University, Pullman

Abstract

Embryos were recovered from super-ovulated rabbits and transferred to beef cattle. Three techniques of embryo transfer were attempted: (a) non-surgical, (b) non-surgical with CO₂ insufflation, using the "Kidde" insufflator and (c) surgical via a paralumbar laparotomy. An apparatus made of three plastic tubes and two long metal needles was used to by-pass the cervix and to transfer the embryos non-surgically into the uterus. Carbon dioxide insufflation through the cervix was essential for successful transfer. This non-surgical technique was inferior to the surgical technique in terms of percent of embryos successfully transferred. Viable rabbit embryos of 16- and 32-cell stages developed to the blastocyst stage in the cow's uterus.

Embryos were recovered from super-ovulated cattle and transferred by laparotomy to the Fallopian tubes or uteri of 1-, 2-, 3- and 4-day pseudopregnant rabbits. Viable cattle embryos of 1- to 8-cell stages failed in most cases to cleave when kept for 48 hr. in the genitalia of pseudopregnant rabbits.

¹ This investigation was supported by a research grant (3031) from the Population Council, Inc., New York, and a research grant (G 9938) from the National Science Foundation.

² Scientific Paper No. 2236, Washington Agricultural Experiment Stations, Pullman, Washington. Project No. 1695.

³ Thanks are due to J. B. Jewell, Ayerst Laboratories, Inc., for a supply of "Equinex" PMS; to R. A. Gessert, The Upjohn Co., for HCG and to R. F. Baker, Abbott Laboratories, for "Vetrophin" and Nembutal. Thanks are due also to R. E. Mauer and W. L. Hunt for technical assistance.

⁴ Present address, National Institute of Animal Industry, Aoba-Cho, Chiba-Shi, Japan.