J. Anim Sci.
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J. Anim Sci. 1962. 21:706-710.
© 1962 American Society of Animal Science
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Multiple-Tube Laboratory Technique for Studying Volatile Fatty Acid Production by Rumen Protozoa1

Wm. C. Christiansen, L. Y. Quinn and Wise Burroughs

Iowa Agricultural and Home Economics Experiment Station, Ames

Abstract

A multiple-tube laboratory technique for studying factors influencing volatile fatty acid production by rumen protozoa in the absence of bacteria was described and illustrated by example. The technique involved harvesting a large mass of protozoa from a rumen fistulated animal, separation of protozoa from bacteria and food particles, and multiple-tube fermentation tests with imposed variables with results assayed by titration of volatile fatty acids following incubation. The technique was illustrated by results with a high degree of repeatability showing a desirable pH of approximately 7.0 for maximum VFA production by rumen protozoa.

Footnotes

1 Journal Paper No. J-4268 of the Iowa Agricultural and Home Economics Experiment Station, Ames, Iowa. Project No. 1208.






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Copyright © 1962 by the American Society of Animal Science.