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Activities of Dried Rumen Microorganisms In Vitro^{1, 2,}

Texas Agricultural Experiment Station^,5, College Station

Abstract

Samples Of Commercial Dried Rumen Preparations And Lyophilized Rumen Fluid Prepared In The Laboratory Were Compared With Fresh Rumen Liquid With Respect To Their Abilities To Digest Cellulose, To Degrade Carboxymethylcellulose (Cmc), To Utilize Urea Nitrogen, And To synthesize riboflavin, niacin, and vitamin ₁₂. None of the commercial preparations or laboratory lyophilized preparations showed effective ability to digest cellulosin vitroduring 24-hour incubation periods. Varying the cultural conditions and adding materials reported to stimulate cellulose digestion by inocula prepared from fresh rumen contents failed to enhance the activity of these preparations. However, all of the dried preparations degraded CMC, thus indicating that organisms attacking this substrate are not necessarily true cellulose digesters. Utilization of urea nitrogen and vitamin synthesis were also much lower by the dried rumen products than by fresh rumen liquor.

¹ Aided by A Grant From Vitamins, Inc., Chicago, Illinois.

² We are Grateful to the Following Companies for Generous Supplies of Dried Rumen Preparations: Vitamins, Inc., Chicago, Illinois; B. A. Lucius Co., Fort Worth, Texas; The Cudahy Laboratories, Omaha, Nebraska; Fort Dodge Laboratories, Fort Dodge, Iowa; and Norden Laboratories, Lincoln, Nebraska.

³ Taken from a thesis submitted by the senior author to the Graduate School of the Agricultural and Mechanical College of Texas in partial fulfillment of the requirements for the degree Master of Science.

⁴ Present address, Alcon Laboratories, Forth Worth, Texas.

⁵ Department of Biochemistry and Nutrition.