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Longevity, Fecundity, Motility, and pH of Diluted and Undiluted Bovine Semen

University of Nebraska

Abstract

The literature on artificial insemination is replete with studies which furnished incidental or secondary observations that led to the discovery of certain useful diluters for semen specimens. Few diluters, however, have been found to be satisfactory for the preservation of the fertilizing capacity of spermatozoa during storage. Some of the various diluters have been successful for the prolonged maintenance of spermatozoan motility. This premise has been accepted by some as a criterion of fecundity of the male germ cells, yet, any perusal of the literature will soon convince the reader that there is a dearth of data regarding the relationship of stored motile spermatozoa in vitro to fecundity. In 1932 Milovanov (4) reported that lecithin extracted from the yolk of hen's egg provided an excellent base for semen diluents. In a recent communication Phillips (6) reported that the survival of bovine spermatozoa was greatly enhanced by storage in a diluter composed of a phosphate buffered solution of the yolk of hen's egg.