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Factors Influencing Motility and Metabolism in Ram Semen^*

University and Missouri, and United States Department of Agriculture Cooperating

Abstract

The preservation or storage of semen between the time of collection and the time of insemination has been in the past and remains at present one of the major problems of artificial insemination. The motility rating and the period of the duration of motility are two criteria accepted as indicative of the viability or state of preservation of the spermatozoa in stored semen. Numerous investigators using a variety of methods have endeavored to prolong the storage period or to increase the motility of spermatozoa after definite periods of storage to a rate above that normally obtained. The most typical of these methods are the use of low storage temperatures and the addition of various diluting media to the semen before storage. In the main these methods for the preservation of spermatozoan motility were suggested by the comparative physiological and biochemical knowledge of other types of cells and the results evaluated in terms of their utility.

^* Contribution from the Department of An. Husb., Mo. Agr. Exp. Sta. Journal Series.