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Preservation of fertilising capacity of horse semen

Cambridge University, England

Abstract

Introduction

The preservation of horse semen presents greater difficulties than that of the bull or ram. The difference probably lies in the degree of dilution with the secretions of the accessory sexual glands as is shown in the following figures.

These figures are subject to considerable variation according to different authorities and according to the methods used in preservation, but the general order is about the same.

According to Milovanov (1934) this effect of dilution is due to the longer time taken by the diluted semen to reach a condition of anabiosis, since in the diluted semen the products of metabolism do not accumulate rapidly and inhibit respiratory activity, but the effect may also be due to the deleterious effect of the secretions themselves, and the "dilution effect" observed by Gray (1928 (a) (b)) may also play a part.

In these experiments an attempt has been made to reproduce artificially in horse semen the condition of high concentration which exists in the semen of the bull and ram, and to replace the deleterious accessory secretions with an artificial medium favourable to the preservation of the spermatozoon.