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Missouri Experiment Station
Abstract
It has been stated that when the percentage of protein in a ration is greatly increased a correspondingly larger quantity of vitamin B must be supplied. Hogan and Pilcher (Mo. Agr. Exp. Sta. Res. Bui. No. 195, 1933) were unable to verify this contention, but they observed, as have others, that rats on high protein diets make considerably larger gains in weight than do those on low protein diets, even though the intake of metabolizable energy is the same. It seemed clear that the failure to make equal gains in weight was due either to a difference in the amount of energy liberated as heat or to a difference in the nature of the gains. The data to be reported were obtained in an effort to decide between these two possibilities. The procedure was practically the same as that of Hogan and Pilcher. Male albino rats were the experimental animals. Of the rations that proved most successful for our purpose the one low in protein contained 6 per cent of casein and 64 per cent of starch. The total amount of protein in this diet, including that furnished by 8 per cent of dried yeast, was approximately 10.0 per cent. The ration high in protein contained 24 per cent of casein and 46 per cent of starch. The total amount of protein in this diet was approximately 26.2 per cent. The proportions of other constituents in these two rations were identical. The animals on the high protein diet secured slightly more metabolizable, and considerably more gross energy, than did those on the low protein diet. The gross energy of the food has been used in our calculations.
1 Paper 85 in the Herman Frasch Foundation Series.
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