J. Anim Sci.
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J. Anim Sci. 1960. 19:1164-1171.
© 1960 American Society of Animal Science

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Quantitative Estimations of Myoglobin and Hemoglobin in Beef Muscle Extracts1, 2,

H. P. Fleming3, T. N. Blumer and H. B. Craig4

North Carolina Agricultural Experiment Station, Raleigh

Abstract

Several methods were used to estimate quantitatively the myoglobin and hemoglobin concentration in beef muscle extracts. Of the methods used, determinations based on the conversion of these pigments in muscle extracts to carbon monoxide compounds provided the most favorable results. This method was tested with solution mixtures of myoglobin and hemoglobin of pre-determined concentrations. The total pigment concentration, as well as percentages of the two component pigments, were in close agreement with the values of the test solution mixtures.


Footnotes

1 Approved for publication as Paper No. 1170 in the Journal Series of North Carolina Agricultural Experiment Station.

2 This work is cooperative with the U. S. Department of Agriculture and is a part of SM-19 Southern Regional Beef Project.

3 From a portion of a thesis presented to the Graduate School, North Carolina State College in partial fulfillment of the requirements for the M.S. degree, 1958.

4 Present address:Department of Animal Husbandry,Michigan State University,East Lansing, Michigan.




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