J. Anim Sci.
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J. Anim Sci. 1960. 19:1143-1149.
© 1960 American Society of Animal Science
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Some Variation in Estrogenic Activity in Fresh and Dried White Clover Clones and the Ladino Variety

E. M. Bickoff1, A. L. Livingston1, A. N. Booth1, C. R. Thompson2, E. A. Hollowell3 and E. G. Beinhart3, 4,

Abstract

Factors affecting the estrogenic activity of white clover clones and the Ladino variety were studied at two different stations. Factors included variety, stage of maturity, age of cutting, season of the year and artificial drying. At the Clemson station, little or no correlation could be found between estrogenic activity and stage of maturity, age of stand, clone or virus infection.

At the Albany station estrogenic activity was found in the Ladino clover sampled periodically from several cuttings grown during a period of over a year, including the "winter" season. There was considerable fluctuation in activity within a given cutting and between cuttings. The estrogenic potency tended to increase with the age of the cutting during the winter season in which the material grew slowly and remained in the vegetative stage. In the early spring, the activity was highest during the budding stage and then decreased greatly as the plant matured. Generally, slight but unpredictable losses of activity occurred during oven-drying of fresh Ladino clover samples.

Footnotes

1 Western Utilization Research and Development Division, Agricultural Research Service, U.S. Department of Agriculture, Albany, Calif. The authors are indebted to Mrs. Ann Gramps for assistance in the preparation of samples for assay and to Mr. A. P. Hendrickson for carrying out the mouse-uterine-weight assays.

2 Present address: University of California, Riverside, Calif.

3 Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture.

4 South Carolina Agricultural Experiment Station, Clemson, South Carolina.






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