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Motility of Boar Spermatozoa as Influenced by Semen Freezing Procedures

Ohio Agricultural Experiment Station and Ohio State University¹, Wooster and Columbus

Abstract

An efficient program of swine artificial insemination may be possible and practicable if improved methods of semen preservetion can be developed. Freezing and storage at sub-zero temperature may be a method of preserving boar semen for prolonged periods.

One of the earliest reports of the freezing of semen was by the Daveport (1897) who reported the survival of human spermatozoa after exposure to a temperature of —17° C. Reports published by Jahnel (1939), Luyet and Hodapp (1938), Hoagland and Pincus (1942), Shaffner (1942) and Polge and Rowson (1952) have shown that several types of mammalian spertozoa are viable after freezing.

In the preparation of semen for cooling, glycerol did not have any detrimental effects on bovine spermatozoa in the dilluted state. In fact, he was able to demonstrate an increase in the conception rate of cattle when 10% gylcerol was added to bull semen diluted with egg yolk citrate.

¹ The paper has been approved by the Station Director.