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Estrogenic Activity of Soybean Forage¹

North Carolina State College,² Raleigh

Abstract

An investigation of green soybean forage was conducted for the purpose of determining if this forage had any estrogenic activity. A crude methanol extract of the forage was fractionated, and the fractions obtained were tested for estrogenic activity by the mouse uterine weight technique. Estrogenic activity was detected in green soybean forage. The estrogenically active substance could be removed from the methanol extract of the plant material with ether; it was not soluble in either benzene or aqueous sodium bicarbonate. From 100 gm. of green soybean forage (fresh weight basis), the estrogenic effect on the uterus of the immature mouse was equivalent to the uterine stimulation produced by approximately 0.03 mcg. of diethylstilbestrol.

¹ Published with the approval of the Director of Research as Paper No. 859 of the Journal Series. The data were taken from a thesis submitted by the senior author in partial fulfillment of the requirements for the Master of Science degree.

² Animal Nutrition Section, Department of Animal Industry.