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In Vitro Volatile Fatty Acid Production from Various Feeds by Bovine Rumen Microorganisms¹

Cornell University,^,4 Ithaca, New York

Abstract

Several common dairy feeds were compared based on their 12-hour production of VFA in vitro. The in vitro method used yielded reproducible results between replicates.

Urea consistently increased volatile fatty acid production in vitro regardless of the substrates used, although in subsequent in vivo experiments it increased VFA production only slightly.

Fresh hand-clipped legume mixed grass caused a greater VFA production than did legume hay. The grass markedly depressed propionic acid formation, however, compared to hay.

Molasses consistently decreased acetic acid production. Beet pulp significantly increased acetic acid production and corn meal increased propionic acid production.

¹ These data were taken from material submitted by W. E. Stewart in partial fulfillment of the requirements for the Doctor of Philosophy degree, Cornell University, Ithaca, New York, 1957.

² Present address: Department of Physiology, New York State Veterinary College, Cornell University, Ithaca, New York.

³ Present address: Dairy Husbandry Department, University of Wisconsin, Madison, Wisconsin.

⁴ Animal Husbandry Department.