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Dilution and Storage of Boar Semen

Illinois Agricultural Experiment Station, Urbana

Abstract

Techniques for handling, diluting and storing boar semen are described. Several different diluters were compared in their ability to sustain sperm motility under conditions in which semen was diluted at the rate of 1 part of semen to 2 parts of diluter. On this basis the most satisfactory diluter consisted of 3 gm. glucose, 0.15 gin. sodium bicarbonate, 30 ml. egg yolk, and 70 ml. of distilled water plus 100,000 units of penicillin and 100 mg. of streptomycin. Maintenance of motility was superior in samples stored at 7° C. than in those stored at 16° C.

¹ The author acknowledges assistance of Neil McCoughlin, Louis James, Pierre Rio, and Gordon Henshaw in the collections of semen and certain analyses. Appreciation is also extended to the Dairy Science Department for permitting the use of the osmometer.