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An Improved Method for the Determination of Metabolic Fecal P¹

University of California^{2, ,3}

Abstract

A method is described for the determination of metabolic fecal phosphorus. This method makes use of P³² labeled calcium phosphate which is injected either subcutaneously or intraperitoneally. Metabolic fecal phosphorus is calculated by comparing the level of P³² in the feces to that in the plasma 24 hours earlier. The daily rate of secretion of metabolic fecal phosphorus appears to be proportional to total fecal phosphorous excretion. Subcutaneous injection of labeled calcium phosphate gives a distinct advantage over both intraperitoneal calcium phosphate and intravenous sodium phosphate. The level of P³² in the plasma (and feces) reaches a nearly constant level within 3 days after the injection and remains essentially unchanged for at least 27 days thereafter.

¹ This study was partially supported by a grant from the United States Atomic Energy Commission.

² Department of Animal Husbandry, Davis.

³ The authors wish to acknowledge the technical assistance of chemist Crawford F. Brown.