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The Influence of Fat, Chlortetracycline, and Stilbestrol on Vitamin A and Carotene Storage in the Liver of Beef Steers¹

State College of Washington³

Abstract

The effect of dietary chlortetracycline, stilbestrol, and fat on liver storage of carotene and vitamin A in steers indicates that:

1. Feeding fat resulted in a highly significant increase of carotene retention, but not vitamin A, in the liver regardless of carotene levels used.
   
2. Neither stilbestrol nor chlortetracycline influenced liver carotene or vitamin A storage.
   

¹ Scientific Paper No. 1474 Washington Agricultural Experiment Stations, Department of Animal Husbandry, Pullman. Project No. 1217.

² Conducted in partial fulfillment for the degree, Doctor of Philosophy in Animal Science.

³ Appreciation is expressed to: Centennial Flouring Mills, Spokane, David Proctor, for mixing a portion of the feed at cost. Mallinckrodt Chemical Works, New York, R. B. Clark, for the stilbestrol used in this test. National Renderers Association, Chicago, John Hamel, Jr., President, for support of this project. American Company, Research Division, New York, R. F. Elliott, for contributing the chlortetracycline used in this test. Ralston Purina Company, Spokane. Mark Hengen, for mixing a portion of the feed and pelleting the rations at cost. Western States Meat Packers Association, Inc., San Francisco, E. B. Forbes, for support of this project. J. G. Darroch and R. E. Christian for aid in statistically analyzing the data.