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Michigan Agricultural Experiment Station4
Abstract
Cellulose digestion by rumen microorganisms in vitro was slightly depressed by 12 ppm. of added cobalt and strongly depressed by 100 ppm. Added vitamin B12 up to 50 meg. per ml. had no appreciable effect on cellulose digestion in vitro.
Feeding high levels of cobalt to a steer eventually resulted in a depression of the in vitro digestion of cellulose by inocula obtained from this animal. The depression was not as great when urea was added to the in vitro fermentation as when it was omitted. Digestion of the cellulose of alfalfa leaf meal in the presence of urea was not depressed.
1 Published with the approval of the Director of the Michigan Agricultural Experiment Station as Journal Article No. 1899.
2 This article is part of a dissertation presented to the faculty of the School for Advanced Graduate Studies of Michigan State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy.
3 Present address: Department of Agricultural Chemistry, Michigan State University, East Lansing.
4 Departments of Dairy and Microbiology and Public Health, East Lansing.
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