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The Influence of Diethylstilbestrol on Certain Blood and Liver Constituents of Lambs¹

University of Wisconsin²

Abstract

Experiments were conducted to determine the influence of diethylstilbestrol implantation on blood and liver composition in feeder lambs. Blood analyses were performed on samples taken from the jugular vein of 20 lambs on the 23rd day of the experiment. Fifteen mg. of diethylstilbestrol were implanted subcutaneously in the ear of one-half of the animals; the other half was not treated and served as controls. The bleeding procedure involved taking samples at random from one-half of the treated and one-half of the control lambs in the a.m. and similarly from the remainder in the p.m.

The estrogen treated lambs had lower hematocrit, and higher levels of plasma free cholesterol and fibrinogen than their controls. Total plasma lipid was higher in the p.m. samples for both treated and control lambs. There was an interaction of estrogen treatment and time of bleeding for non-protein nitrogen, globulin, total protein, and phospholipid.There were no statistically significant differences between treated and control animals for these constituents in the a.m. blood samples. In the p.m. blood samples, the treated animals had a significantly lower quantity of non-protein nitrogen and phospholipid and a significantly higher quantity of globulin and total protein than their controls. There were no significant effects of estrogen treatment or time of bleeding on plasma glucose, albumin, ester cholesterol, total cholesterol, or neutral fat.

Liver samples from two estrogen treated and two control lambs receiving each of five different rations were obtained at the time of slaughter. A significantly greater amount of total liver dry matter and a significantly smaller amount of liver ester cholesterol was found in the treated lambs. Estrogen treatment did not affect the liver content of percentage dry matter, glycogen, protein, phospholipid, free cholesterol, total cholesterol, neutral fat, or total lipid. Ration had no effect on the liver constituents studied.

¹ Published with the approval of the Director of the Wisconsin Agricultural Experiment Station, Madison.

² Departments of Animal Husbandry, Biochemistry, and Genetics (paper No. 544), Madison, Wisconsin.