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University of Wisconsin
Abstract
Motility and fertility variations of rabbit spermatozoa were induced by experimentally altering pH and osmotic pressure. Four different buffer systems (mono- and disodium phosphate, mono- and dipotassium phosphate, sodium citrate and citric acid and potassium citrate and citric acid) have been used as sperm diluents. The first study was designed to determine for each buffer system the optimum molality and pH for motility as well as hypertonic and hypotonic concentrations, and pH values which would support some motility but at a low level. In the second experiment these particular variations in pH and molality were studied to determine their effects on the relation between motility and fertility. The differences in fertility in the experimental diluents from the fertility in the control diluents per unit difference in motility were found to differ significantly from one experimental diluent to another.These results showed that motility in vitro and fertility are phenomena which may or may not be affected similarly by an environmental factor.
1 Published with the approval of the director of the Agricultural Experiment Station. Paper No. 540 from the Department of Genetics.
2 On study leave from Faculty of Agriculture, Ibrahim University, Egypt.
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