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Distribution of Intravenously Injected Radio-Active Phosphorus (P³²) among Sheep Tissues¹

University of California^,4

Abstract

THE use of artificially radioactive isotopes as tracers in mineral metabolism studies has been previously described in experiments with swine (Smith et al., 1951). This paper reports the utilization of plasma inorganic phosphorus (as traced by radioactive phosphorus) by the soft tissues of sheep at different ages, and at different times after injection of the tracer.

Method

Three groups of sheep of different ages (approximately one, four, and ten months) were selected from the Animal Husbandry flock. A description of these animals is given in table 1. An isotonic buffered solution, containing P³² labeled sodium phosphate, was injected intravenously. In the larger animals, an intravenous catheter was installed in the jugular vein for the injection (Ralston et al., 1949). In the smaller animals where this was not possible, a bleeding needle with the catheter attached was used to locate and penetrate the vein. The syringe containing the radioactive solution was then connected to the free end of the catheter, and the drawing back of blood at the beginning and end of the injection was used to check the proper position of the needle for the injection.

¹ This work was supported by the U. S. Atomic Energy Commission.

² Postdoctoral Fellow of the Atomic Energy Commission; present address: Division of Poultry Husbandry, University of California, Davis, California.

³ The authors gratefully acknowledge the technical assistance of T. N. Chernikoff.

⁴ Division of Animal Husbandry, Davis, California.