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Factors Influencing the In Vitro Digestion of Cellulose by Rumen Liquor in the Presence of an Antiseptic

The Ohio State University

Abstract

Cellulose digestion may be demonstrated by the in vitro action of rumen liquor on filter paper in the presence of toluene, through the accumulation of reducing substances.

Toluene proved to be the most effective antiseptic to prevent further changes of the reducing substances which are first produced in cellulose digestion. The optimum pH lies between 4.53 and 7.35. Phosphate and glycine buffers appeared to be more favorable than borate or citrate.Anaerobic conditions are not essential. The cellulolytic activity of rumen liquor, with or without added buffer, decreases on standing in the presence of toluene, and ceases after about 48 hours.

The factors which promote digestion of cellulose by rumen liquor do not appear to pass through a Berkfeld filter.

The loss of cellulolytic activity of rumen liquor on dialysis cannot be restored by the addition of various vitamin mixtures, yeast, beef and alfalfa extracts, bovine blood, evaporated dialyzate, or boiled or filtered rumen liquor. Some of the activity may be restored by the addition of solutions of ashed alfalfa hay and of rumen liquor.