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The Effect of the Method of Making Semen Smears upon the Number of Morphologically Abnormal Spermatozoa

Cornell University²

Abstract

The determination of the number of morphologically abnormal spermatozoa in a sample of semen is widely used as a diagnostic aid in identifying certain cases of male sterility, both in man and the lower animals. It is stated that reliable estimates may be made of the potential fertility of males by this means. Williams and Kingsbury (Ig:m), Williams and Savage (I925), Mason(1929) , Moench and coworkers (1930, 1931), and McKenzie and Phillips (1933, 1934) have all emphasized the importance of this technique in the assay of potential male fertility.

In investigations of bull semen, however, we have noted a wide variability in the counts of certain types of morphologically abnormal spermatozoa. This fact appeared to be true not only with respect to different bulls but also appeared to be associated with the manner in which the semen was handled during and after collection and the techniques employed in the preparation of the slides for staining.

The question arises as to whether the variability noted was due to an inherent difference in the samples of semen examined or whether the methods of handling and methods of preparation of slides were responsible for the variability.

¹ Now Animal Husbandman, Agricultural Experiment Station, Rio Piedras, Puerto Rico.

² Department of Animal Husbandry, Ithaca, N. Y.