J. Anim Sci.
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J. Anim Sci. 1942. 1:149-154.
© 1942 American Society of Animal Science
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Effect of Diluters and Storage upon Fecundity1 of Bovine Semen

Gravers K. L. Underbjerg, H. P. Davis and R. E. Spangler2

University of Nebraska

Abstract

Fresh semen samples were treated and used for inseminations as follows: I, no treatment (control); II, diluted with fresh egg yolk buffer; III, diluted with stored egg yolk buffer; IV, diluted with autoclaved milk. Other samples V, VI, VII, and VIII receiving the same series of treatments respectively were stored prior to insemination. A total of 931 cows were inseminated in these eight groups of which 492 conceived. Conception percentages in the various groups were as follows: I, 63.5; II, 54.5; III, 42.9; IV, 46.5; V, 36.7; VI, 25.9; VII, 38.5; VIII, 30.8.

A test of significance employing chi-square values indicated that the treatments accorded the fresh and stored semen in the various groups did not cause a significant lowering of the conception percentages from the controls. Conversely the stored groups as a whole showed a highly significant departure from the conception percentage in the fresh control group despite the retention of a high degree of motility of the spermatozoa.

It is concluded that the treatments had little beneficial effect on fecundity, but that during storage other factors are in operation which cause loss of the fertilizing capacity of bovine spermatozoa regardless of retention of motility.

Footnotes

1 Submitted with the approval of the Director as paper No. 300 Journal Series, Nebraska Agricultural Experiment Station.

2 Dairy Husbandry Department, Lincoln, Nebraska and Purina Mills Experimental Farm, Gray Summit, Missouri.

Acknowledgment is made of a grant in aid for this study by the American Dairy Cattle Club.






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