Table 6. Concentrations (as-is basis) of vitamin D₃, 25-hydroxyvitamin D₃ (25-OH D₃), and 1,25-dihydroxyvitamin D₃ (1,25-(OH)₂ D₃) in LM steak, liver, kidney, and serum of cull cows fed control, supplemental 25-hydroxyvitamin D₃ (25-OH D₃), or manipulated dietary cation-anion difference diet plus supplemental 25-OH D₃ (DCAD+25-OH D₃)

Treatment Item Control 25-OH D3 DCAD+25-OH D3 SEM P-value Vitamin D3, ng/g     LM 1.23 1.05 0.93 0.10 0.75     Liver 11.4 10.9 10.3 3.5 0.88     Kidney 13.0 13.8 14.9 2.6 0.77     Serum, ng/mL 4.015 4.832 4.281 0.79 0.95 25-OH D3, ng/g     LM 1.83a 4.53b 4.01b 0.24 0.04     Liver 4.5a 17.3b 17.9b 2.6 0.001     Kidney 4.2a 8.7b 7.3b 2.0 0.01     Serum, ng/mL 88a 383b 488c 72 0.0001 1,25-(OH)2 D3, pg/g     LM 24.0a 22.7a 17.3b 3.4 0.002     Liver 14.3a 16.2a 19.6b 1.9 0.0001     Kidney 14.7a 19.2b 19.9b 2.6 0.001     Serum, ng/mL 10a 25b 34c 6.7 0.0001 a–cWithin a row, means lacking a common superscript letter differ (P < 0.05).